Use of the comet test to assess DNA damage in children with ataxia-telangiectasia and their relatives

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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Published on: March 24, 2020

L Moreno Galarraga1, J L Santos Pérez, M C Ramirez-Tortosa

  • 1Department of Pediatrics, University Hospital Virgen de las Nieves, Granada, Spain.

Journal of Investigational Allergology & Clinical Immunology
|May 2, 2008
PubMed
Abstract

No abstract available in PubMed .

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A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...

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