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Updated: Jul 5, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
Published on: May 16, 2022
Ultrabroadband multiplex CARS microspectroscopy and imaging using a subnanosecond supercontinuum light source in the
Masanari Okuno1, Hideaki Kano, Philippe Leproux
1Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo, Tokyo 113-0033, Japan.
Abstract:
Subnanosecond supercontinuum (SC) has been generated by a 1,064 nm microchip laser combined with a photonic crystal fiber. The ultrabroadband (>2,000 cm(-1)) SC has facilitated multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy in the spectral range from 1,000 to 3,000 cm(-1) with lateral and depth spatial resolution of 0.9 and 4.6 microm, respectively. A clear CARS image of a Nicotiana tabacum L. cv. Bright Yellow 2 cell has been obtained with high vibrational contrast.
