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Updated: Jul 5, 2026

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
Published on: March 28, 2008
The actin binding protein, fesselin, is a member of the synaptopodin family
Mechthild M Schroeter1, Brent Beall, Hans W Heid
1Department of Biochemistry & Molecular Biology, Brody School of Medicine at East Carolina University, 600 Moye Blvd, Greenville, NC 27834, USA. schroeterm@ecu.edu
Abstract:
Fesselin is a natively unfolded protein that is abundant in avian smooth muscle. Like many natively unfolded proteins, fesselin has multiple binding partners including actin, myosin, calmodulin and alpha-actinin. Fesselin accelerates actin polymerization and bundles actin. These and other observations suggest that fesselin is a component of the cytoskeleton. We have now cloned fesselin and have determined the cDNA derived amino acid sequence. We verified parts of the sequence by Edman analysis and by mass spectroscopy. Our results confirmed fesselin is homologous to human synaptopodin 2 and belongs to the synaptopodin family of proteins.
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