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Related Experiment Videos

Interferences in immunoassay.

Jill Tate1, Greg Ward

  • 1Department of Chemical Pathology, Queensland Health Pathology Service, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia. jill_tate@health.qld.gov.au

The Clinical Biochemist. Reviews
|May 7, 2008
PubMed
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Immunoassay interference from various substances can lead to inaccurate patient results and incorrect treatment. Laboratories and physicians must collaborate to detect and manage these interferences effectively.

Area of Science:

  • Clinical Chemistry
  • Immunology
  • Laboratory Medicine

Background:

  • Immunoassay interference arises from substances altering analyte concentration or antibody binding.
  • Endogenous (e.g., autoantibodies) and exogenous factors (e.g., lipaemia, pre-analytical variation) can cause interference.
  • Interference affects various analytes including hormones, tumor markers, and cardiac troponin.

Purpose of the Study:

  • To highlight the mechanisms and impact of immunoassay interference.
  • To emphasize the importance of detecting and reporting interference.
  • To stress the need for laboratory-physician collaboration in managing interference.

Main Methods:

  • Review of known interfering substances and their mechanisms.
  • Discussion of analytical challenges and detection strategies.

Related Experiment Videos

  • Emphasis on communication protocols between laboratories and physicians.

    • Main Results:

    • Interference can cause falsely elevated or decreased analyte concentrations, leading to misdiagnosis.
    • Prevalence varies; blocking agents reduce but do not eliminate interference.
    • New immunoassays may have higher interference rates.
    • Interference impacts a broad range of clinically significant analytes.

    Conclusions:

    • Effective management requires robust laboratory detection and reporting processes.
    • Physician communication of clinical suspicion is crucial for identifying discordant results.
    • Collaboration and clear communication are essential to prevent patient harm from immunoassay interference.