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Hydrogen peroxide modulation of the respiratory burst of human neutrophils
J S Winn1, J Guille, J M Gebicki
1School of Biological Sciences, Macquarie University, North Ryde, NSW, Australia.
Biochemical Pharmacology
|January 1, 1991
Abstract:
Addition of micromolar concentrations of hydrogen peroxide (H2O2) to human neutrophils resulted in a dose-dependent luminol-enhanced chemiluminescent response. Pretreatment of neutrophils with micromolar concentrations of H2O2 altered their response to the surface acting stimulants serum-treated zymosan (STZ) and formyl-methionyl-leucyl-phenylalanine (fMLP), but not to the intracellular stimulant phorbol myristate acetate (PMA). The alterations were partially reversible by catalase, but exacerbated by superoxide dismutase. These results suggest a modulatory role for H2O2 in the respiratory burst of neutrophils.