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Updated: Jul 5, 2026

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
Published on: May 31, 2019
Detection of microcystin-metal complexes by using cryospray ionization-Fourier transform ion cyclotron resonance mass
Kazunori Saito1, Yoshihisa Sei, Shinichi Miki
1Bruker Daltonics K.K., 3-9 A-6F Moriya-cho, Kanagawa-ku, Yokohama, Kanagawa, Japan. kazunori.saito@bruker-daltonics.jp
Abstract:
Complexes of microcystins (MCs) and metal ions were detected with mass spectrometry. To observe the MCs-metals complexes, CryoSpray ionization ion source was developed and it was equipped on a commercial Fourier transform ion resonance mass spectrometer. Mixtures of two MCs and five metals were applied to Fourier transform ion cyclotron resonance mass spectrometry coupled with CryoSpray. The analyses showed complexes of MC-LR-Fe(II), -Zn, -Cu, -Mg, MC-RR-Fe(II), -Zn, -Cu, and -Mg but MC-LR-Fe(III) and MC-RR-Fe(III) were not observed. Present study suggested that MCs may play roles in metal ion uptake and/or accumulation in the algal cells.
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