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Related Experiment Videos

Binary system for regulating transgene expression in mice: targeting int-2 gene expression with yeast GAL4/UAS

D M Ornitz1, R W Moreadith, P Leder

  • 1Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, MA 02115.

Proceedings of the National Academy of Sciences of the United States of America
|February 1, 1991
PubMed
Summary

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A novel binary transgenic system enables targeted gene activation in offspring via a simple genetic cross. This system uses yeast regulatory sequences (UAS) and the GAL4 activator for precise control of transgene expression, leading to specific phenotypes.

Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Transgenic systems are crucial for studying gene function.
  • Controlling transgene expression in specific tissues or developmental stages remains a challenge.
  • Existing methods often lack precise spatiotemporal control.

Purpose of the Study:

  • To develop a binary transgenic system for inducible transgene activation in progeny.
  • To enable targeted expression of a specific gene (int-2) using a genetic cross.
  • To investigate the phenotypic consequences of targeted int-2 gene overexpression.

Main Methods:

  • Creation of two transgenic mouse strains: 'target' (carrying a UAS-controlled transgene) and 'transactivator' (expressing GAL4 under a specific promoter).

Related Experiment Videos

  • Crossbreeding of target and transactivator strains to generate bigenic offspring.
  • Analysis of transgene expression and resulting phenotypes in bigenic progeny.

    • Main Results:

    • The binary system successfully activated the silent transgene in bigenic offspring.
    • High levels of the target int-2 gene were expressed in organs where GAL4 was active.
    • Bigenic offspring exhibited a dominant int-2 phenotype, including hyperplasia and hypertrophy, leading to male sterility.

    Conclusions:

    • The developed binary transgenic system provides a powerful tool for conditional gene activation in a heritable manner.
    • This system allows for precise control over transgene expression patterns through promoter selection in the transactivator.
    • The study demonstrates the utility of this system for studying gene function and developmental processes, exemplified by the int-2 phenotype.