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Gut ischemia/reperfusion induced acute lung injury is an alveolar macrophage dependent event

Luciana Borsoi Moraes1, Abel Hiroshi F Murakami, Belchor Fontes

  • 1Laboratory of Medical Investigation of the 3rd Division of Surgical Clinic, Hospital das Clinicas, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.

The Journal of Trauma
|May 13, 2008
PubMed
Abstract

Insights

Eliminating lung alveolar macrophages (AMs) prevented acute lung injury (ALI) following intestinal ischemia/reperfusion (I/R) in rats. This indicates AMs are crucial mediators of ALI after intestinal I/R.

Area of Science:

  • Physiology
  • Immunology
  • Pathology

Background:

  • Alveolar macrophages (AMs) are implicated in lung acute lung injury (ALI) following lung ischemia/reperfusion (I/R).
  • The role of AMs in ALI after intestinal I/R remains unclear.
  • This study investigates AMs' role in ALI subsequent to intestinal I/R.

Purpose of the Study:

  • To determine if AM elimination impacts ALI development after intestinal I/R in rats.
  • To elucidate the specific contribution of AMs to intestinal I/R-induced ALI.

Main Methods:

  • Male Wistar rats were treated with clodronate-liposomes (CLOD-LIP) to deplete AMs, liposomes (LIP), or no treatment (UNTREAT).
  • Animals underwent intestinal ischemia/reperfusion (I/R), laparotomy (LAP), or served as controls (CTR).
  • Acute lung injury (ALI) was quantified using the Evans blue dye (EBD) method to measure lung vascular permeability.

Main Results:

  • Intestinal I/R significantly increased ALI in LIP and UNTREAT groups compared to sham and control groups.
  • CLOD-LIP treatment significantly reduced ALI in the I/R group compared to LIP and UNTREAT groups.
  • These findings demonstrate that I/R induces ALI, which is preventable by AM depletion.

Conclusions:

  • Alveolar macrophages (AMs) play a critical role in the pathogenesis of ALI following intestinal ischemia/reperfusion (I/R).
  • Depletion of AMs effectively mitigates intestinal I/R-induced ALI.
  • Targeting AMs may represent a therapeutic strategy for preventing ALI in the context of intestinal I/R injury.

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