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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Related Experiment Video

Updated: Jul 5, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Next-generation high-density self-assembling functional protein arrays.

Niroshan Ramachandran1, Jacob V Raphael, Eugenie Hainsworth

  • 1Harvard Institute of Proteomics, Harvard Medical School, 320 Charles Street, Cambridge, Massachusetts 02141, USA.

Nature Methods
|May 13, 2008
PubMed
Summary
This summary is machine-generated.

Researchers created a high-density protein microarray using the nucleic acid programmable protein array (NAPPA) method. This technique allows for high-throughput study of protein function by displaying thousands of proteins efficiently.

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Last Updated: Jul 5, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
13:22

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Proteomics

Background:

  • Protein microarrays are essential tools for studying protein function and interactions.
  • Current methods for protein display on microarrays can be limited in scale and efficiency.
  • The nucleic acid programmable protein array (NAPPA) concept offers a promising approach for in situ protein production and capture.

Purpose of the Study:

  • To develop and validate a high-density self-assembling protein microarray.
  • To enable high-throughput screening of protein functions.
  • To improve the efficiency and reproducibility of protein display on microarrays.

Main Methods:

  • Development of a self-assembling protein microarray based on the NAPPA concept.
  • Immobilization of complementary DNA (cDNA) templates for in situ protein production.
  • Arraying up to 1,000 unique human cDNAs for protein display.

Main Results:

  • Achieved high yields of protein expression and capture.
  • Demonstrated minimal variation and good reproducibility across the array.
  • Successfully displayed thousands of unique proteins from immobilized cDNA templates.

Conclusions:

  • The developed high-density protein microarray is a powerful tool for large-scale functional proteomics.
  • This method facilitates high-throughput experimental approaches for studying protein function.
  • The NAPPA-based approach offers a robust and reproducible platform for protein microarray development.