Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conditional gene expression and knockdown using lentivirus vectors encoding shRNA.

Jolanta Szulc1, Patrick Aebischer

  • 1Neurosciences Institute, Swiss Federal Institute of Technology Lausanne, Lausanne, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urolithin A improves muscle strength, exercise performance, and biomarkers of mitochondrial health in a randomized trial in middle-aged adults.

Cell reports. Medicine·2022
Same author

Effect of Urolithin A Supplementation on Muscle Endurance and Mitochondrial Health in Older Adults: A Randomized Clinical Trial.

JAMA network open·2022
Same author

Astrocyte-targeting RNA interference against mutated superoxide dismutase 1 induces motoneuron plasticity and protects fast-fatigable motor units in a mouse model of amyotrophic lateral sclerosis.

Glia·2022
Same author

Direct supplementation with Urolithin A overcomes limitations of dietary exposure and gut microbiome variability in healthy adults to achieve consistent levels across the population.

European journal of clinical nutrition·2021
Same author

The mitophagy activator urolithin A is safe and induces a molecular signature of improved mitochondrial and cellular health in humans.

Nature metabolism·2020
Same author

Evolution of the neurochemical profiles in the G93A-SOD1 mouse model of amyotrophic lateral sclerosis.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2018
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This study presents a drug-inducible lentiviral system for controlled gene expression and knockdown. The robust system offers precise control in various cell types and in vivo models for research and potential gene therapy applications.

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Mammalian Genetics

Background:

  • Drug-inducible systems are crucial for controlling gene expression in research and therapy.
  • Lentivector-based systems offer a versatile platform for conditional gene manipulation.
  • Previous methods lacked the robustness and versatility needed for broad applications.

Purpose of the Study:

  • To provide a detailed methodology for constructing and utilizing a drug-inducible lentiviral system.
  • To enable drug-controllable expression of transgenes and gene knockdown via small hairpin RNAs (shRNAs).
  • To offer quantitative analyses of the system's performance in vitro and in vivo.

Main Methods:

  • Development of a lentivector-based conditional gene expression system.

Related Experiment Videos

  • Application of the system for transgene expression and shRNA-mediated gene knockdown.
  • In vitro studies in various mammalian cell lines and in vivo studies in mouse models.

    • Main Results:

    • The lentiviral system demonstrated robust and tightly controlled transgene expression.
    • Effective gene knockdown of endogenous genes was achieved using shRNAs.
    • The system proved versatile across different cell types and in vivo models, including the central nervous system and xenotransplanted tumors.

    Conclusions:

    • The developed lentiviral system provides a powerful tool for drug-inducible gene control.
    • This technology has significant potential for advancing genetic research, translational studies, and gene therapy.
    • The methodology and analyses presented facilitate the application of this system in diverse biological contexts.