Related Experiment Video
Updated: Jul 5, 2026

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat
Published on: July 26, 2015
[Preparation and clinical application of an intelligent determination and analysis system for glomerular filtration
Xi-Xin Wu1, Min Qu, Min-Jian Feng
1Department of Nephrology of The Second People's Hospital of Guangdong Province, Guangzhou 510317, Guangdong, China.
Objective:
To prepare an intelligent determination and analysis system for renal glomerular filtration rate(GFRBMAS), and to explore its value in clinical setting.
Methods:
GFRBMAS was prepared by programming with VB 6.0 software. GFR of 79 inpatients suffering from the different diseases was determined accurately by using clearance rate of (99)Tc(m)-diethylene triamine pentaacetic acid (DTPA) (Tc-GFR). The serum creatinine (SCr), blood urea nitrogen (BUN), serum uric acid (Uric), serum calcium (Ca) and serum phosphorus (P) were determined with both GFRBMAS and 7170S automatic biochemistry determination apparatus (ititachi), and the result of GFR was compared with that determined by using GFRBMAS and 7170S automatic biochemical determination apparatus. At the same time GFR was determined by using Robert formula (GFRBMAS-GFR, Robert-GFR), and creatinine clearance rate was calculated with Cockcroft/Gault formula (CG-CCr). All the results were compared and analyzed.
Results:
No significant difference of SCr, BUN, Uric, Ca and P values determined by two methods. Robert-GFR and CG-CCr values were significantly lower than Tc-GFR value in the normal renal function group and the renal insufficiency group (P<0.01) and that of GFRBMAS-GFR was close to that of Tc-GFR and relative analysis showed that the values of GFRBMAS-GFR, Robert-GFR, CG-CCr showed significantly positive correlation with that of Tc-GFR, but negative correlation with values of SCr and BUN (P<0.05 or P<0.01).
Conclusion:
GFRBMAS-GFR, Robert-GFR and CG-CCr could all reflect GFR with accuracy to certain extent and GFRBMAS-GFR can take the place of Tc-GFR in clinical setting.
Related Concept Videos
Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate
Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration
Glomerular Filtration Rate and its Regulation
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
Renal Clearance
Renal clearance refers to the volume of plasma cleared of a specific substance, such as creatinine, per unit of time. To measure clearance, urine samples are collected over a 24-hour period during each bladder voiding, followed by a single blood sample at the...
Renal Drug Excretion: Glomerular Filtration
Drugs gain access to the kidney via the renal artery, which progressively branches off into afferent arterioles.
Factors Affecting Renal Clearance: Renal Impairment
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...

