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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Related Experiment Video

Updated: Jul 5, 2026

Technique to Target Microinjection to the Developing Xenopus Kidney

Technique to Target Microinjection to the Developing Xenopus Kidney

Published on: May 3, 2016

A functional screen for genes involved in Xenopus pronephros development.

Jun-ichi Kyuno¹, Karine Massé, Elizabeth A Jones

¹Department of Biological Sciences, Warwick University, Gibbet Hill Road, Coventry CV4 7AL, UK.

Mechanisms of Development

|May 13, 2008

Summary

Researchers identified 31 novel genes crucial for Xenopus pronephros development using a functional screen. Overexpression of selected genes like Sox7 and Dnajc15 caused specific pronephric abnormalities, validating the screen

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Last Updated: Jul 5, 2026

Technique to Target Microinjection to the Developing Xenopus Kidney

Technique to Target Microinjection to the Developing Xenopus Kidney

Published on: May 3, 2016

Functional Cloning Using a Xenopus Oocyte Expression System

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Published on: February 2, 2016

Area of Science:

Developmental Biology
Comparative Anatomy
Genetics

Background:

The Xenopus pronephros, a simple yet functionally analogous larval kidney, serves as a valuable model for studying vertebrate kidney development.
Its basic organization mirrors that of more complex kidneys, making it ideal for genetic screens.

Purpose of the Study:

To conduct a functional genetic screen to identify novel genes involved in Xenopus pronephros development.
To characterize the roles of specific identified genes in pronephric morphogenesis and function.

Main Methods:

A gain-of-function screen was performed by injecting mRNA from a Xenopus cDNA library into X. laevis eggs.
Sib-selection identified clones causing abnormal pronephric phenotypes, with gene function assessed via in situ hybridization and antibody staining.
Five candidate genes (Sox7, Cpeb3, P53csv, Mecr, Dnajc15) with specific pronephric expression were selected for further analysis.

Main Results:

Out of 768 screened cDNA clones, 31 genes (approximately 4%) significantly affected pronephric marker expression.
Most identified genes showed expression in the pronephros and possess functions relevant to developmental processes.
Overexpression of the five selected genes resulted in distinct temporal and spatial pronephric abnormalities.

Conclusions:

The functional screening strategy effectively identified novel genes essential for pronephros development in Xenopus.
This approach is robust for discovering genes involved in late developmental events, providing insights into vertebrate kidney formation.