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Updated: Jul 5, 2026
08:05
Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
Following protein kinase acivity by electrochemical means and contact angle measurements
Agnieszka Wieckowska1, Di Li, Ron Gill
1Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
Chemical Communications (Cambridge, England)
|May 14, 2008
Abstract:
The electrochemical analysis of the protein kinase, casein kinase, is accomplished by the voltammetric response of Ag(+) ions associated with the phosphorylated product; the sensing surface is regenerated by the cleavage of the phosphorylated product with alkaline phosphatase, and the phosphorylation/de-phosphorylation processes are monitored by XPS and contact angle measurements.
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