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Trans-active factors controlling the IL-2 gene in adult human T-cell subsets

A Mouzaki1, R H Zubler, A Doucet

  • 1Haematology Geneva University Hospital 25, rue Micheli-du-Crest Geneva 4 CH-1211 Switzerland.

Mediators of Inflammation
|January 1, 1992
PubMed

IL-2 secretion in total or subsets of PHA/PMA-stimulated PBMC-derived human T-lymphocytes was monitored and found to be largely due to CD4(+)CD8(-) cells. The presence and functional state of transcription factors (TF) was assessed by protein-DNA interaction assays and functional transactivation experiments in the Xenopts oocyte system, modulating IL-2 transcription by injection of proteins. The results reveal that CD4(+)CD8(-) cells contain both, functional silencer in their resting, and positive TF in their activated states while the CD4(+)CD8(-) group contains only non-functional positive TF. This demonstrates that the on/off switch of IL-2 transcription is based on the same mechanism in primary T-lymphocytes of mouse spleen and in peripheral human CD4(+)CD8(-) cells.

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