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Determination of phytase activity in feed: interlaboratory study
Gisele Gizzi1, Peter Thyregod, Christoph von Holst
1European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Retieseweg 111, B-2440 Geel, Belgium.
Journal of AOAC International
|May 15, 2008
Summary
A new method for determining phytase activity in feed was validated through an interlaboratory study. The validated method demonstrated reliable performance, comparable to existing phytase assays.
Area of Science:
- Enzymology
- Analytical Chemistry
- Animal Nutrition
Background:
- Phytase is a crucial enzyme in animal feed, enhancing phosphorus utilization.
- Accurate determination of phytase activity is essential for feed formulation and quality control.
- Existing methods may have limitations in validating diverse phytase products.
Purpose of the Study:
- To validate a new analytical method for quantifying phytase activity in animal feed.
- To assess the performance characteristics, including precision and accuracy, of the new method.
- To demonstrate the method's suitability for various phytase products.
Main Methods:
- An interlaboratory study involving multiple laboratories was designed.
- The method relies on measuring inorganic phosphate released from phytate substrate.
- Assay precision was evaluated under repeatability, intermediate precision, and reproducibility conditions.
Main Results:
- Relative standard deviation for repeatability (RSDr) ranged from 2.2% to 10.6%.
- Relative standard deviation for reproducibility (RSDR) ranged from 5.4% to 15%.
- The method's performance profile was comparable to existing phytase assay methods.
Conclusions:
- The validated method is suitable for determining phytase activity in feed samples.
- The method provides reliable and reproducible results across different laboratories.
- This validated method offers a robust tool for assessing phytase enzyme activity in diverse feed products.
