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Column reversed-phase high-performance liquid chromatographic method for simultaneous determination of rabeprazole
Shweta Sadanand Sabnis1, Dhavale Nilesh Dnvandev, Vijay Yeshawantrao Jadhav
1All India Shri Shivagi Memorial Society's College of Pharmacy, Department of Pharmaceutical Analysis, Kennedy Rd, Pune 411001, Maharashtra, India.
Abstract:
A new, simple column reversed-phase high-performance liquid chromatographic (HPLC) method for simultaneous determination of rabeprazole sodium (RAB) and domperidone (DOM) in a combined tablet dosage form has been developed and validated. Determination was performed using a Jasco HPLC system with a HiQ SiL octadecylsilane (C18) column (250 x 4.6 mm id), acetonitrile-0.1 M ammonium acetate (50 + 50, v/v) mobile phase, and paracetamol as an internal standard. The detection was performed using a UV detector set at 280 nm. The method was validated with respect to linearity, accuracy, precision, and robustness. Beer's law was obeyed in the concentration range of 1.0-10.0 and 0.5-5.0 microg/mL for RAB and DOM, respectively. The method has been successfully applied for the analysis of drugs in a pharmaceutical formulation.
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