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Published on: April 23, 2019
High-performance thin-layer chromatographic determination of rabeprazole sodium and domperidone in combined dosage
Santosh Vilashchand Gandhi1, Samina I Khan, Rashmi T Jadhav
1AISSMS College of Pharmacy, Department of Pharmaceutical Analysis, Pune, India. santoshvgandhi@rediffmail.com
Abstract:
A new, simple, high-performance thin-layer chromatographic method for determination of rabeprazole sodium (RAB) and domperidone (DOM) in combined tablet dosage form has been developed and validated. The mobile phase was toluene-acetone-methanol (4.5 + 4.5 + 0.5, v/v/v) with UV detection at 285 nm. The retention factors for RAB and DOM were found to be 0.53 +/- 0.12 and 0.32 +/- 0.20. The method was validated with respect to linearity, accuracy, precision, and robustness. Beer's law was obeyed in the concentration range of 50-800 ng/band for both RAB and DOM. The method has been successfully applied for the analysis of drugs in a pharmaceutical formulation.
