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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Extraction of Extracellular Vesicles from Whole Tissue
09:03

Extraction of Extracellular Vesicles from Whole Tissue

Published on: February 7, 2019

Electromembrane extraction of peptides.

Marte Balchen1, Léon Reubsaet, Stig Pedersen-Bjergaard

  • 1School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, Norway.

Journal of Chromatography. A
|May 16, 2008
PubMed
Summary

Electromembrane extraction (EME) rapidly extracts peptides using a supported liquid membrane and electric potential. This novel method offers efficient peptide separation, with recoveries depending on peptide structure.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Biochemistry

Background:

  • Peptide extraction is crucial for analysis and purification.
  • Traditional methods can be time-consuming and complex.
  • Novel, rapid extraction techniques are needed.

Purpose of the Study:

  • To demonstrate the first-time use of electromembrane extraction (EME) for rapid peptide recovery.
  • To investigate the efficiency and parameters influencing EME of peptides.
  • To assess the potential of EME as a valuable tool for peptide analysis.

Main Methods:

  • Utilized electromembrane extraction (EME) with a supported liquid membrane (SLM).
  • Employed a hollow fiber setup with aqueous sample and acceptor solutions.
  • Applied a 50 V potential across the SLM to drive peptide migration.

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  • Optimized SLM composition (1-octanol and di(2-ethylhexyl) phosphate) and electrolyte concentrations.

    • Main Results:

    • Achieved rapid extraction of eight model peptides within 5 minutes.
    • Demonstrated enrichment factors up to 11 times.
    • Identified peptide polarity and ionization as key factors influencing extraction recovery.
    • Established optimal conditions using specific electrolyte concentrations and agitation.

    Conclusions:

    • Electromembrane extraction is a viable and rapid technique for peptide separation.
    • EME offers a promising alternative for peptide extraction in various applications.
    • Further research can optimize EME for specific peptide targets and complex matrices.