Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Suppression of macrophage enriched miRNA 210-3p improves cardiac fibrosis and cardiac function following myocardial infarction.

Clinical hemorheology and microcirculation·2026
Same author

Potentiated Maturation of hPSC-Derived Dopaminergic Neurons via Convergent Genetic and Small Molecule Modulation.

Tissue engineering and regenerative medicine·2026
Same author

MicroRNA-30c-1-3p Alleviates Hypoxia-Induced Cardiomyocyte Dysfunction via <i>Tnrc6a</i> Targeting.

Biomedicines·2026
Same author

Proangiogenic and Collagen-Promoting Effects of a 70% Ethanol Extract of <i>Grateloupia angusta</i> in Cutaneous Wound Models.

International journal of molecular sciences·2026
Same author

Retinoic acid signaling regulates astrocyte reactivity by modulating MAPK/NF-κB pathways and mitochondrial integrity.

Neurochemistry international·2026
Same author

Telomere Attrition-Induced Senescence in Human Pluripotent Stem Cell-Derived Astrocytes: Distinct Cellular and Functional Characteristics.

Journal of cellular physiology·2025

Related Experiment Video

Updated: Jul 5, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Chemicals that modulate stem cell differentiation.

Ki-Chul Hwang1, Ji Young Kim, Woochul Chang

  • 1Cardiovascular Research Institute, Department of Physiology, and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.

Proceedings of the National Academy of Sciences of the United States of America
|May 16, 2008
PubMed
Summary
This summary is machine-generated.

Researchers screened protein kinase inhibitors to control stem cell differentiation. Two isoquinolinesulfonamide derivatives effectively differentiated rat mesenchymal stem cells (MSCs) into chondrocytes and mouse embryonic stem cells (ESCs) into dopaminergic neurons.

More Related Videos

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation
06:55

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Published on: July 17, 2019

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
11:29

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

Published on: July 17, 2016

Related Experiment Videos

Last Updated: Jul 5, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation
06:55

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Published on: July 17, 2019

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
11:29

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

Published on: July 17, 2016

Area of Science:

  • Biochemistry
  • Cell Biology
  • Developmental Biology

Background:

  • Cell fate is regulated by complex signaling pathways, many of which remain poorly understood.
  • Protein kinases play crucial roles in modulating these signaling pathways and influencing cell fate decisions.
  • Identifying chemical regulators of protein kinases is essential for understanding and manipulating stem cell differentiation.

Purpose of the Study:

  • To screen known protein kinase inhibitors for their ability to modulate stem cell differentiation.
  • To identify specific chemical compounds that can effectively alter the differentiation pathways of mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs).

Main Methods:

  • Screened 41 characterized inhibitors targeting six major protein kinase subfamilies.
  • Assessed the impact of these inhibitors on the differentiation rates of rat MSCs and mouse ESCs.
  • Identified and characterized the most effective compounds based on observed differentiation changes.

Main Results:

  • Several protein kinase inhibitors induced noticeable changes in stem cell differentiation rates.
  • Two isoquinolinesulfonamide derivatives demonstrated significant efficacy in directing stem cell differentiation.
  • Specifically, these compounds promoted rat MSC differentiation into chondrocytes and mouse ESC differentiation into dopaminergic neurons.

Conclusions:

  • Protein kinase inhibitors can be utilized as tools to control stem cell differentiation pathways.
  • Isoquinolinesulfonamide derivatives show promise as effective agents for targeted stem cell differentiation.
  • This study provides a foundation for developing novel chemical strategies to guide cell fate determination.