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Signal processing by the HOG MAP kinase pathway.

Pascal Hersen1, Megan N McClean, L Mahadevan

  • 1FAS Center for Systems Biology and School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

Proceedings of the National Academy of Sciences of the United States of America
|May 16, 2008
PubMed
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Researchers measured the information capacity of the high osmolarity glycerol (HOG) pathway in yeast. This signaling pathway acts as a low-pass filter, integrating signals more effectively when they change slowly.

Area of Science:

  • Cellular signaling
  • Systems biology
  • Biophysics

Background:

  • Cellular signaling pathways transmit environmental information for appropriate cellular responses.
  • The information capacity, or bandwidth, of these pathways is crucial for cellular function.
  • The high osmolarity glycerol (HOG) pathway in Saccharomyces cerevisiae is a well-studied model for cellular stress response.

Purpose of the Study:

  • To quantify the information capacity and bandwidth of the yeast HOG signaling pathway.
  • To investigate how pathway architecture influences its information processing capabilities.
  • To measure in vivo reaction rates within the HOG pathway.

Main Methods:

  • Development of a microfluidic device for precise control of single-cell environments across various frequencies.

Related Experiment Videos

  • Measurement of the HOG pathway's response to dynamic osmotic stimuli.
  • Kinetic analysis of specific pathway components, including the Ssk1 and Ste11 branches.

    • Main Results:

    • The HOG pathway functions as a low-pass filter, accurately transmitting slow environmental changes while integrating rapid ones.
    • Previously uncharacterized in vivo reaction rate bounds for the HOG pathway were determined.
    • The Ssk1 component exhibits rapid signal integration, while the Ste11 component shows limitations in speed.

    Conclusions:

    • The HOG pathway's bandwidth is dependent on its specific architecture and component kinetics.
    • The study provides a framework for measuring signaling pathway kinetics and information capacity.
    • The developed experimental techniques are applicable to a broader range of cellular signaling studies.