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Multiscale modeling of protein uptake patterns in chromatographic particles
1Center for Molecular and Engineering Thermodynamics, Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA. lenhoff@udel.edu
Langmuir : the ACS Journal of Surfaces and Colloids
|May 17, 2008
Summary
A new diffusion model explains protein uptake into porous materials. This model predicts protein diffusivity based on adsorption conditions, aiding optimization for chromatography separations.
Area of Science:
- Physical Chemistry
- Chemical Engineering
- Biophysics
Background:
- Protein uptake into porous adsorbents exhibits complex mechanisms.
- Previous models have not fully captured the observed transition in uptake behavior.
Purpose of the Study:
- To present a parallel diffusion model explaining the observed transition in protein uptake mechanisms.
- To develop a predictive relationship for apparent protein diffusivity.
Main Methods:
- Developed a mesoscopic diffusion model.
- Incorporated microscopic models for surface diffusion (hopping) and adsorption (Gibbs surface excess).
Main Results:
- Derived a relationship for apparent protein diffusivity as a function of adsorption conditions.
- The model provides predictive capabilities using measurable physical quantities.
Conclusions:
- The developed model successfully explains the apparent transition in protein uptake.
- This approach can optimize conditions for preparative protein chromatography.
