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Updated: Jul 5, 2026
Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
Published on: August 2, 2011
Development and characterization of a nucleic acid probe for avian reoviruses
1Department of Veterinary Medicine, National Chung Hsing University Taichung, Taiwan 403.
Abstract:
A radiolabelled probe (S4-18), corresponding to 542 base pairs of the S4 genome segment of avian reovirus (ARV), was used in dot blot hybridization assays. The lowest limit of purified viral RNA detected was similar among three ARV strains and was 0.2 ng. The probe also detected nine heterologous strains of ARV RNA prepared directly from cell cultures, indicating that it had a broad specificity. In addition, the probe detected ARV RNA in tendon tissues of birds from commercial broiler raising farms following dot blot hybridization.
