Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
06:18

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Published on: July 11, 2025

SNP analysis using CataCleave probes.

John J Harvey1, Steven R Brant, Jay R Knutson

  • 1Excimus Biotech, Inc., 8510 Corridor Road, Savage, MD 20763, USA. harvey@excimus.com

Journal of Clinical Laboratory Analysis
|May 20, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-Cell Imaging of Mitochondrial Metabolism and Remodeling in C2C12 Murine Skeletal Muscle Cells upon Differentiation.

International journal of molecular sciences·2026
Same author

IBD Care Across Diverse Patient Populations: Changing the Narrative.

Digestive diseases and sciences·2026
Same author

In vitro effects of Taurine on Taxol-induced mitochondrial dysfunction.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same author

gDAIS: A Global Analysis Application for Obtaining Confidence in FLIM.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same author

Effect of Load on Non-Muscle Myosin 2 Paralog Filaments in a Biomimetic Contractile Actin Array.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Postoperative Ileum Transcriptomics Implicate Sex-Biased Mechanisms in Crohn's Disease Recurrence.

Gastroenterology·2025
Same journal

Changes of CEA Diagnostic Performance Under the New Reference Interval Stratified by Age and Sex.

Journal of clinical laboratory analysis·2026
Same journal

Investigation of Specific IgG-Secreting Cells in Congenital Toxoplasmosis: The TOXODIAG Study.

Journal of clinical laboratory analysis·2026
Same journal

Development and Analytical Validation of a Laboratory-Customized TaqMan-MGB Probe Method for CYP2C19 Genotyping.

Journal of clinical laboratory analysis·2026
Same journal

Heavy Metal Mixture Exposure and Insulin Resistance in U.S. Adults: The Mediating Role of Systemic Inflammation.

Journal of clinical laboratory analysis·2026
Same journal

A New Diagnostic Approach Using the NLR/LMR Ratio in the Differential Diagnosis of Neck Masses: Hematologic Evaluation of Patients With Cervical Lymphadenopathy.

Journal of clinical laboratory analysis·2026
Same journal

Elevated Hemoglobin A<sub>2</sub>: A Molecular Revisited, and Implications to β-Thalassemia Screening.

Journal of clinical laboratory analysis·2026
See all related articles

CataCleave probes offer real-time detection of genetic variations like single nucleotide polymorphisms (SNPs) using a novel DNA-RNA-DNA structure. This method enables sensitive and specific identification of mutations and methylated DNA.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Förster resonance energy transfer (FRET) is commonly used for fluorescence-based detection.
  • Ribonuclease H (RNase H) is an enzyme that cleaves RNA in DNA-RNA hybrids.
  • Single nucleotide polymorphisms (SNPs) are common genetic variations that can impact health.

Purpose of the Study:

  • To develop and validate catalytically cleavable fluorescence probes (CataCleave) for detecting genetic variations.
  • To assess the probes' ability to identify single nucleotide polymorphisms (SNPs), insertions, deletions, and methylated DNA.
  • To demonstrate the probes' utility in homogeneous reactions under isothermal and temperature cycling conditions.

Main Methods:

  • Design of chimeric DNA-RNA-DNA probes (CataCleave) utilizing Förster resonance energy transfer (FRET) for signal generation.

More Related Videos

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

Related Experiment Videos

Last Updated: Jul 5, 2026

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
06:18

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Published on: July 11, 2025

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

  • Utilizing ribonuclease H (RNase H) for cleavage of the RNA moiety upon target binding and hybridization.
  • Testing probe performance in detecting SNPs in the IGF-2 and NOD2/CARD15 genes, a deletion in the amelogenin gene, and methylated DNA.

    • Main Results:

    • CataCleave probes successfully detected SNPs, insertions, deletions, and methylated DNA with high specificity.
    • Probe cleavage and fluorescence recovery were significantly reduced with single-base mismatches, indicating high discrimination.
    • Cleavage rates were found to be proportional to the target DNA concentration.

    Conclusions:

    • CataCleave probes represent a novel and effective method for real-time, homogeneous detection of various genetic variations, including SNPs.
    • The probe design allows for modification for multiplexing and high-throughput SNP assays.
    • This technology offers a sensitive and specific approach for genetic analysis.