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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: Jul 5, 2026

Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition
09:37

Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition

Published on: August 18, 2022

Skin imaging with reflectance confocal microscopy.

Kishwer S Nehal1, Dan Gareau, Milind Rajadhyaksha

  • 1Memorial Sloan Kettering Cancer Center, New York, NY 10022, USA.

Seminars in Cutaneous Medicine and Surgery
|May 20, 2008
PubMed
Summary
This summary is machine-generated.

Confocal microscopy provides high-resolution, real-time imaging of living skin, offering a noninvasive alternative to traditional biopsies. This advanced technique aids in diagnosing skin lesions and understanding skin conditions.

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Area of Science:

  • Dermatology
  • Medical Imaging
  • Optical Technology

Background:

  • Confocal microscopy offers high-resolution, real-time imaging of biological tissues.
  • It provides contrast comparable to conventional histology without tissue processing.
  • Recent advancements have focused on its application in dermatology for skin imaging.

Purpose of the Study:

  • To highlight the significance of confocal microscopy in dermatology.
  • To explore its noninvasive capabilities for in-vivo skin examination.
  • To outline its potential clinical and research applications in skin imaging.

Main Methods:

  • Utilizing confocal microscopy for noninvasive, real-time imaging of skin.
  • Comparing imaging quality with conventional histology.
  • Applying the technology to various dermatological scenarios.

Main Results:

  • Confocal microscopy achieves high resolution and contrast in living skin.
  • It allows for virtual examination of skin without biopsy.
  • Demonstrated utility in differentiating benign and malignant lesions.

Conclusions:

  • Confocal microscopy represents a significant advancement in dermatological imaging.
  • It offers a noninvasive method for skin assessment, reducing the need for biopsies.
  • Potential applications span diagnosis, treatment monitoring, and research in dermatology.