Related Experiment Video
Updated: Jul 5, 2026
Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models
Published on: September 18, 2018
[A morphological study with serial histological slices on the normal and abnormal gubernacula in newborn male mice]
Qiang Liu1, Xue-Wu Jiang, Zhong-Xian Chen
1Department of Pedicatic Surgery, the Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Objective:
To explore the feasibility of serial slices microscopic histological investigation for the elaborate evaluation of reproductive system malformations.
Methods:
Newborn male mice prenatally exposed to different doses of subcutaneously given diethylstilbestrol (DES) from gestational day 9 to 17 were treated by fixing parts of the abdomen in situ and setting them to transected serial slices. All the slices were stained, studied under the microscope and serially recorded by software. The gubernaculum was morphologically analyzed and its location and size were measured.
Results:
Morphologically, the gubernaculum could be identified clearly, its structure inhomogeneous from proximal to distal and dissymmetric from right to left. The environmental estrogen produced different effects on the morphology of the gubernaculum in different parts and most obviously affected its length.
Conclusion:
Prenatal exposure to environmental estrogen has evident and general effects on the gubernacular development of newborn male mice. The morphological study with serial histological slices gives a precise and systematic evaluation of genital malformations.
