Related Experiment Video
Updated: Aug 8, 2026

Production of Double-stranded DNA Ministrings
Published on: February 29, 2016
The inhibition of restriction endonucleases due to Z-DNA in negatively supercoiled plasmid
E A Lesnik1, Zh A Bes'chetnikova, R N Maslova
1Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Abstract:
Plasmid pGC20 containing the (dGC)9 insert in SmaI recognition site has been used to study the inhibition of cleavage by different restriction endonuclease due to Z-DNA formation in (dCG)10 sequence of the negatively supercoiled plasmid. Data obtained indicate the different sensitivity of restriction endonucleases to DNA conformational perturbations resulted from the Z-DNA formation. Therefore, the inhibition of DNA cleavage by a particular restriction endonuclease cannot serve as a criterion for the estimation of the length of B-Z junctions in circular supercoiled DNAs.
Related Concept Videos
DNA Topoisomerases
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...
Restarting Stalled Replication Forks
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Single-Strand DNA Binding Proteins
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Inhibitors of Bacterial DNA Synthesis

