Related Experiment Video
Updated: Jul 5, 2026

Structure-Guided Design and Development of Novel Cyclophilin A Inhibitors and Ganoderiol-F Derivatives: An In-Silico Approach
Published on: June 23, 2026
Chiral selector with multiple hydrogen-bonding sites in a macrocyclic cavity
Tadashi Ema1, Daisuke Tanida, Kyoko Sugita
1Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University, Tsushima, Okayama, Japan. ema@cc.okayama-u.ac.jp
New chiral stationary phases (CSPs) were developed for high-performance liquid chromatography (HPLC). These CSPs effectively resolved diverse chiral compounds using various mobile phases, including organic solvents and supercritical CO2.
Area of Science:
- Analytical Chemistry
- Separation Science
- Organic Chemistry
Background:
- Chiral compounds require specialized separation techniques.
- Developing efficient chiral stationary phases (CSPs) is crucial for chromatography.
- Macrocyclic compounds offer unique structural features for chiral recognition.
Purpose of the Study:
- To synthesize novel chiral macrocycles for use as CSPs.
- To evaluate the enantioseparation capabilities of the synthesized CSPs in HPLC.
- To explore the impact of mobile phase composition on resolution efficiency.
Main Methods:
- Synthesis of chiral macrocycles with hydrogen bonding sites.
- Covalent bonding of macrocycles to silica gel to create CSPs.
- High-performance liquid chromatography (HPLC) analysis of chiral compounds like benzoin and Co(acac)3.
- Optimization of mobile phase composition, including organic solvents and supercritical CO2.
Main Results:
- The synthesized CSPs demonstrated excellent enantiomeric resolution abilities.
- Various chiral analytes, including benzoin and tris(acetylacetonato)cobalt(III) (Co(acac)3), were successfully separated.
- Mobile phase flexibility was observed, with organic solvents, acetonitrile (MeCN), methanol (MeOH), and supercritical carbon dioxide (CO2) enabling complete enantiomer resolution in certain cases.
Conclusions:
- Novel chiral macrocycle-based CSPs offer high efficiency for chiral separations in HPLC.
- The developed CSPs provide versatile separation capabilities with adaptable mobile phases.
- These findings contribute to advancements in enantioselective chromatography.
More Related Videos
06:35Construction and Systematical Symmetric Studies of a Series of Supramolecular Clusters with Binary or Ternary Ammonium Triphenylacetates
Published on: February 15, 2016
08:25Development of Heterogeneous Enantioselective Catalysts using Chiral Metal-Organic Frameworks (MOFs)
Published on: January 17, 2020
Related Concept Videos
Prochirality
Molecules with Multiple Chiral Centers
Regioselectivity and Stereochemistry of Acid-Catalyzed Hydration
Stereoisomerism of Cyclic Compounds
Regioselectivity of Electrophilic Additions to Alkenes: Markovnikov's Rule
The hydrohalogenation of an unsymmetrical alkene can yield two haloalkane products, depending on which vinylic carbon takes up the halogen. However, one product usually predominates, where hydrogen adds to the vinylic carbon bearing the...
Chirality at Nitrogen, Phosphorus, and Sulfur
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...