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Updated: Jul 5, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Two-color planar laser-induced fluorescence thermometry in aqueous solutions

G Andrew Robinson1, Robert P Lucht, Normand M Laurendeau

  • 1School of Mechanical Engineering, Purdue University, West Lafayette, IN 47907, USA.

Applied Optics
|May 22, 2008
PubMed

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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.