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Updated: Jul 5, 2026
Anticancer Metal Complexes: Synthesis and Cytotoxicity Evaluation by the MTT Assay
Published on: November 10, 2013
Predictive value of MTT assay as an in vitro chemosensitivity testing for gastric cancer: one institution's
Bin Wu1, Jin-Shui Zhu, Yi Zhang
1Department of Gastroenterology, Shanghai Sixth Hospital Affiliated to Shanghai Jiaotong University, Yishang Road 600, Shanghai 200223, China.
Aim:
To investigate the predictive clinical value of in vitro 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay for directing chemosensitivity in patients with gastric cancer.
Methods:
Results of a total of 353 consecutive patients with gastric cancer treated with MTT-directed chemotherapy or physician's empirical chemotherapy from July 1997 to April 2003 were reviewed and analyzed retrospectively.
Results:
The overall 5-year survival rate of MTT-sensitive group (MSG) and control group (CG) was 47.5% and 45.1%, respectively. The results of subgroup analysis with Cox proportional-hazards model were favorable for the MSG-sensitive group. However, no statistically significant difference in survival rate was observed between the two groups.
Conclusion:
Individualized chemotherapy based on in vitro MTT assay is beneficial, but needs to be confirmed by further randomized controlled trials.
