Related Experiment Video
Updated: Jul 5, 2026

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
Published on: October 26, 2016
Separation of chitosan oligomers by immobilized metal affinity chromatography
Frantz Le Dévédec1, Laurent Bazinet, Alexandra Furtos
1Department of Chemistry & Center BioMed, Université du Québec à Montréal, CP 8888, Succursale Centre Ville Montréal (Québec), H3C 3P8, Canada.
Abstract:
A novel approach for chitosan oligosaccharide (COS) separation by immobilized metal affinity chromatography (IMAC) based on the differences in the interactions of chelated copper (II) ions with various COS (dimers, trimers, tetramers) is described. Polyhydroxylic chromatographic supports (agarose CL-6B and silica) were functionalized with various chelating functions such as iminodiacetate (IDA), carboxymethyl-aspartate (CM-Asp) and tris(carboxymethyl)ethylenediamine (TED). The COS retention capacities of the columns were between 2 and 6 mg/cm(3), depending on the chelating group. The COS were separated and/or enriched up to 95% for dimer and trimer and 90% for the tetramer, with yields of 60-95%.
Related Concept Videos
Extraction: Advanced Methods
Affinity Chromatography
Ion-Exchange Chromatography
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Ion Exchange

