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Updated: Jul 5, 2026
12:21
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
Published on: January 7, 2019
HSV as a vector in vaccine development and gene therapy
Peggy Marconi1, Rafaela Argnani, Elena Berto
1Department of Experimental and Diagnostic Medicine, Section of Microbiology; University of Ferrara, Ferrara, Italy. mcy@unife.it
Human Vaccines
|May 24, 2008
Summary
Herpes simplex virus (HSV) genetics knowledge enables the creation of HSV-1 vectors for gene therapy and cancer treatment. These vectors offer potential for targeted delivery and prophylaxis against infections.
Area of Science:
- Genetics and Molecular Biology
- Virology
- Biotechnology
Background:
- Herpes simplex virus (HSV) is a significant human pathogen with a dual lifestyle involving lytic and latent infections.
- Extensive knowledge of HSV genetics and molecular biology has paved the way for therapeutic applications.
Purpose of the Study:
- To review the design, construction, and applications of HSV-1-based vectors.
- To discuss the potential and limitations of different HSV-1 vector classes.
Main Methods:
- Classification of HSV-1 vectors into three distinct categories: replication-competent attenuated, replication-incompetent recombinant, and defective helper-dependent (amplicons).
- Review of current literature on HSV-1 vector development and applications.
Main Results:
- HSV-1 vectors show promise for delivering genes to the nervous system.
- Applications include selective cancer cell destruction, prophylaxis against infections, and targeted tissue/organ infection.
- Three classes of HSV-1 vectors (attenuated, recombinant, amplicons) have been developed.
Conclusions:
- HSV-1-based vectors represent a versatile platform for various biomedical applications.
- Further research is needed to overcome current limitations and fully realize the potential of these vectors.
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