Related Experiment Video
Updated: Jul 5, 2026

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds
Published on: October 18, 2018
Electrochemical analysis of the alanine phenylthiohydantoin derivative by cathodic stripping voltammetry
C Vilaseca1, M C Quintana, J Vicente
1Departamento Química Analítica y Análisis Instrumental, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
Abstract:
A square-wave cathodic stripping voltammetry method for alanine determination as its phenylthiohydantoin (PTH-alanine) derivative is developed. To this end, all the chemical and instrumental variables affecting the determination of PTH-alanine are optimized. From studies of the mechanisms governing the electrochemical response of PTH-alanine, it was concluded that it is an electrochemically irreversible system with a diffusive-adsorptive reduction phenomenon. Under optimal conditions, the variation of analytical signal (I(p)) with PTH-alanine concentration is linear in the 2.4x10(-8)-4.8x10(-7) M range, with a LOD of 1.2x10(-8) M and a LOQ of 4.2x10(-8) M, a RSD (%) less than 11%, and a E(r) (%) less than 10%. The optimized method was applied to the determination of PTH-alanine obtained from a synthetic protein after Edman reaction and the results were corroborated by high-performance liquid chromatography with UV detection.
Related Concept Videos
Voltammetry: Stripping Methods
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
Voltammetric Techniques: Linear-Scan (E vs Time)
Voltammetric Techniques: Cyclic Voltammetry
Electrogravimetric Analysis: Overview
To test the completeness of the...
Voltammetric Techniques: Pulse Voltammetry
Voltammetry: Overview
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
