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Detection of an endothelin-1-binding protein complex by low temperature SDS-PAGE
T Takasuka1, I Horii, Y Furuichi
1Department of Molecular Genetics, Nippon Roche Research Center, Kamakura, Japan.
Biochemical and Biophysical Research Communications
|April 15, 1991
Abstract:
We found that the complex of ET-1 and its binding protein was stable enough to be separated by SDS-PAGE when electrophoresis was run at a low temperature. Cross-linking was not necessary for the detection of [125I]-ET-1 and its binding protein complex by autoradiography. This simple method could be used in qualitative (estimation of apparent molecular weight of ET-1 binding protein) and quantitative (determination of relative content of ET-binding protein) analysis of the ET-binding protein complex. ET-binding protein complexes of various animal species and organs were investigated by this method.