Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Low levels of IgG2 and pneumococcal antibodies as predictors of benefit from IgG replacement in IgG subclass deficiency.

Journal of human immunity·2026
Same author

Glial multicellular programs reveal distinct patient stratification in Parkinson's disease.

Research square·2026
Same author

Spatially resolved T cell receptor diversity mapping uncovers variability of the cancer immune microenvironment.

EBioMedicine·2026
Same author

Microfluidic toolbox using padlock probes and rolling circle amplification for direct detection and genotyping of viral RNA.

RSC advances·2026
Same author

Composite proteomic and metabolomic plasma biomarkers for detection of colorectal, lung and ovarian cancers.

Molecular cancer·2026
Same author

Retrospective evaluation of mandibular third molars using simulated low-dose cone-beam computed tomography: A comparative image quality study.

Imaging science in dentistry·2026

Related Experiment Video

Updated: Jul 5, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Ligation-based molecular tools for lab-on-a-chip devices.

Jonas Melin1, Jonas Jarvius, Chatarina Larsson

  • 1Department of Genetics and Pathology, Rudbeck Laboratory, University of Uppsala, SE 75185 Uppsala, Sweden.

New Biotechnology
|May 28, 2008
PubMed
Summary
This summary is machine-generated.

Molecular diagnostics on microfluidic devices offer early disease detection. Developing suitable molecular assays is key for user-friendly point-of-care diagnostics, overcoming current limitations.

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
11:05

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

Related Experiment Videos

Last Updated: Jul 5, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
11:05

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Microfluidics

Background:

  • Molecular diagnostics enable early disease detection and accurate follow-up.
  • Microfluidic devices promise rapid, user-friendly analyses for point-of-care settings.
  • Limited progress in launching microfluidic diagnostic devices has been observed.

Purpose of the Study:

  • To identify limitations in developing molecular assays for microfluidic devices.
  • To explore molecular tools for enhancing lab-on-a-chip diagnostic capabilities.
  • To facilitate the advancement of point-of-care molecular diagnostics.

Main Methods:

  • Discussion of challenges in establishing molecular assays for microfabricated formats.
  • Review of requirements for molecular assays: broad analyte detection (DNA, RNA, proteins), sensitivity, and specificity.
  • Exploration of novel molecular tools suitable for microfluidic applications.

Main Results:

  • Difficulty in establishing suitable molecular assays is a significant limiting factor.
  • Microfluidic assays must handle diverse molecules including DNA, RNA, and modified proteins.
  • High sensitivity and specificity are critical for effective molecular diagnostics.

Conclusions:

  • Overcoming assay development challenges is crucial for microfluidic diagnostic success.
  • New molecular tools can enhance the capabilities of lab-on-a-chip devices.
  • Advancements in molecular assays will drive the adoption of point-of-care diagnostics.