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Updated: Jul 5, 2026
Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
Published on: October 20, 2019
[Function of F10 gene, a novel hydatidiform mole-related gene: a preliminary study]
Xiao-min Cao1, Zhan-jun Pang, Song Quan
1Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Objective:
To study the function of F10 gene, a novel hydaditiform mole-related gene.
Methods:
A549 cell line was transfected with the F10 gene of forward or reverse sequence or with the empty vector, respectively. The cellular mRNA was extracted after 24 h of transfection to screen for the differentially expressed genes among the 3 transfected and the control cells using differential display-polymerase chain reaction (ddPCR).
Results:
The bands representing differentially expressed genes were amplified from the cells, and the products were linked to T-Vector for sequence analysis. Several genes were screened by Blasting and their expressions were confirmed by fluorescent quantitative PCR.
Conclusion:
F10 gene is functionally related to cell proliferation and apoptosis.
