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Dynamic component chemiluminescent sensor for assessing circulating polymorphonuclear leukocyte activity of
Daria Prilutsky1, Boris Rogachev, Marina Vorobiov
1Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
Abstract:
Recurrent bacterial peritonitis is a major complication in peritoneal dialysis (PD) patients, which is associated with polymorphonuclear leukocyte (PMN) functional changes and can be assessed by a chemiluminescent (CL) reaction. We applied a new approach of a dynamic component chemiluminescence sensor for the assessment of functional states of PMNs in a luminol-amplified whole-blood system. This method is based on the evaluation of CL kinetic patterns of stimulated PMNs, while the parallel measurements of intracellular and extracellular production of reactive oxygen species (ROS) from the same sample can be conducted. Blood was drawn from diabetic and nondiabetic patients during follow-up, and during peritonitis. Healthy medical personnel served as the control group. Chemiluminescence curves were recorded and presented as a sum of three biological components. CL kinetic parameters were calculated, and functional states of PMNs were assessed. Data mining algorithms were used to build decision tree models that can distinguish between different clinical groups. The induced classification models were used afterward for differentiating and classifying new blind cases and demonstrated good correlation with medical diagnosis (84.6% predictive accuracy). In conclusion, this novel method shows a high predictive diagnostic value and may assist in detection of PD-associated clinical states.
Insights
A novel chemiluminescence sensor assesses polymorphonuclear leukocyte (PMN) function in peritoneal dialysis (PD) patients. This method accurately predicts PD-associated clinical states, aiding early diagnosis.
Area of Science:
- Biomedical Engineering
- Clinical Chemistry
- Immunology
Background:
- Recurrent bacterial peritonitis is a significant complication for peritoneal dialysis (PD) patients.
- Peritonitis is linked to altered polymorphonuclear leukocyte (PMN) function.
- Chemiluminescence (CL) reactions can assess PMN activity.
Purpose of the Study:
- To introduce a dynamic component chemiluminescence sensor for evaluating PMN function in PD patients.
- To assess the diagnostic potential of CL kinetic patterns in distinguishing clinical states.
- To correlate sensor data with medical diagnoses.
Main Methods:
- Utilized a luminol-amplified whole-blood system with a dynamic component chemiluminescence sensor.
- Measured intracellular and extracellular reactive oxygen species (ROS) production.
- Analyzed CL kinetic patterns and calculated parameters from stimulated PMNs.
- Employed data mining and decision tree models for classification.
Main Results:
- The sensor assessed PMN functional states by analyzing CL kinetic patterns.
- Decision tree models achieved 84.6% predictive accuracy in classifying clinical groups.
- The method demonstrated a strong correlation with medical diagnoses.
Conclusions:
- The novel dynamic component chemiluminescence sensor offers high predictive diagnostic value.
- This method can assist in the early detection of PD-associated clinical states.
- It provides a valuable tool for monitoring PMN function in PD patients.
