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Related Experiment Video

Updated: Jul 4, 2026

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Published on: February 13, 2019

Angiogenesis in adipose tissue.

Ebba Brakenhielm¹, Yihai Cao

¹Rouen Medical University, Rouen, France.

Methods in Molecular Biology (Clifton, N.J.)

|June 3, 2008

Summary

Angiogenesis, the formation of new blood vessels, is crucial for fat tissue expansion. Suppressing this process may offer a new strategy for treating obesity.

Area of Science:

Physiology
Vascular Biology
Metabolic Disease

Background:

Adipose tissue exhibits significant plasticity, expanding or contracting throughout adult life.
Fat mass expansion relies on angiogenesis, the development of new blood vessels.
Angiogenesis suppression presents a potential therapeutic avenue for obesity.

Purpose of the Study:

To describe methodologies for assessing angiogenesis within adipose tissue.
To provide technical procedures for quantifying blood vessel formation in fat depots.

Main Methods:

Detailed description of two distinct technical procedures.
Focus on the assessment of angiogenesis in adipose tissue samples.

Main Results:

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Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

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Last Updated: Jul 4, 2026

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

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Published on: November 17, 2018

The study outlines specific technical protocols.
These procedures enable the evaluation of angiogenesis in adipose tissue.

Conclusions:

Accurate assessment of angiogenesis in adipose tissue is critical.
The described methods can support research into obesity and related metabolic disorders.