Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 4, 2026

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment
09:13

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Published on: April 4, 2017

Laser-illuminated nanohole arrays for multiplex plasmonic microarray sensing.

Antoine Lesuffleur1, Hyungsoon Im, Nathan C Lindquist

  • 1Laboratory of Nanostructures and Biosensing, Department of Electrical and Computer Engineering, University of Minnesota, Twin Cities, Minneapolis, MN 55455, USA.

Optics Express
|June 4, 2008
PubMed
Summary

This study introduces nanohole arrays for surface plasmon resonance (SPR) imaging, enhancing sensitivity and enabling label-free molecular interaction analysis in microarrays without prisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective Growth of Bulk-Like Perovskite in Plasmonic Nanoholes for Enhanced Two-Photon-Excited Emission.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Hydrogel-based multiplexed high-risk human papillomavirus DNA/RNA detection for cervical cancer screening.

Biosensors & bioelectronics: X·2026
Same author

Molecular Analysis of Single Tumor-Derived Extracellular Vesicles with Improved Robustness and Accuracy.

Analytical chemistry·2026
Same author

Microfluidic pulp platform with vascular integration to evaluate biofunctional material.

Biomaterials·2026
Same author

Spatially Controlled Capture and Site-Resolved Analysis of Single Extracellular Vesicles.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Do drugs with biliary toxicity cause cholangiocarcinoma?

bioRxiv : the preprint server for biology·2026

Area of Science:

  • Nanophotonics and Biosensing
  • Optical Metamaterials
  • Surface Chemistry

Background:

  • Surface Plasmon Resonance (SPR) imaging is a key technique for label-free, real-time molecular interaction analysis.
  • Conventional SPR typically requires a prism coupling system, limiting its application in high-throughput microarray formats.
  • Enhancing SPR sensitivity and simplifying instrumentation are crucial for broader adoption.

Purpose of the Study:

  • To demonstrate a novel approach for SPR imaging using nanohole arrays.
  • To investigate the potential of laser-illuminated nanohole sensors for high-throughput molecular characterization.
  • To assess the performance of nanohole sensors in a microarray format.

Main Methods:

  • Fabrication of periodic nanohole arrays on a gold surface.

More Related Videos

Trapping of Micro Particles in Nanoplasmonic Optical Lattice
07:20

Trapping of Micro Particles in Nanoplasmonic Optical Lattice

Published on: September 5, 2017

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers
09:33

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

Published on: March 21, 2025

Related Experiment Videos

Last Updated: Jul 4, 2026

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment
09:13

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Published on: April 4, 2017

Trapping of Micro Particles in Nanoplasmonic Optical Lattice
07:20

Trapping of Micro Particles in Nanoplasmonic Optical Lattice

Published on: September 5, 2017

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers
09:33

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

Published on: March 21, 2025

  • Illumination of nanohole arrays with a laser source to excite surface plasmons (SPs).
  • Measurement of changes in laser transmission due to molecular binding events on the nanohole sensors.

    • Main Results:

    • Demonstrated SPR imaging using laser-illuminated nanohole arrays, eliminating the need for a prism.
    • Achieved a significant change (>35%) in laser transmission upon formation of a self-assembled monolayer.
    • Successfully measured binding kinetics in parallel from a 5x3 microarray of nanohole sensors.

    Conclusions:

    • Nanohole arrays coupled with laser illumination offer a promising platform for prism-free SPR imaging.
    • This technology significantly enhances detection sensitivity and enables high-throughput analysis of molecular interactions.
    • The nanohole sensor array approach holds potential for advanced microarray-based diagnostics and research.