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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 26, 2013
Th2 differentiation is unaffected by Jagged2 expression on dendritic cells.
Connie M Krawczyk1, Jie Sun, Edward J Pearce
1Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Journal of Immunology (Baltimore, Md. : 1950)
|June 5, 2008
Summary
Jagged2 expression on dendritic cells (DCs) was thought to promote Th2 immune responses. However, this study found Jagged2 is neither sufficient nor required for Th2 cell differentiation, challenging previous hypotheses.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Dendritic cells (DCs) instruct adaptive immune responses.
- The Jagged-Notch signaling pathway is crucial for cell-cell communication.
- Jagged Notch ligands on DCs are implicated in T helper 2 (Th2) cell differentiation.
Purpose of the Study:
- To investigate the role of Jagged2 expression by dendritic cells in inducing Th2 cell differentiation.
- To determine if Jagged2 is sufficient or required for Th2 responses.
Main Methods:
- Correlating Jagged1 and Jagged2 expression with DC-mediated Th2 induction.
- Utilizing retrovirally delivered small interfering RNA to suppress Jagged2 expression.
- Overexpressing Jagged2 in DCs.
- Assessing Th2 cell differentiation in vitro and in vivo.
Main Results:
- Jagged2 expression correlated with DC ability to induce Th2 responses, increasing with helminth antigens and decreasing with TLR agonists.
- Suppression of Jagged2 in DCs did not impair Th2 cell differentiation.
- Enhanced Jagged2 expression in DCs did not boost Th2 cell responses.
Conclusions:
- Jagged2 expression by dendritic cells is not sufficient for Th2 cell differentiation.
- Jagged2 expression by dendritic cells is not required for Th2 cell differentiation.
- The role of Jagged ligands in instructing Th2 responses requires further investigation.
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