Related Experiment Video
Updated: Jul 4, 2026

Extraction of High Molecular Weight DNA from Microbial Mats
Published on: July 7, 2011
Millimeter-scale genetic gradients and community-level molecular convergence in a hypersaline microbial mat
Victor Kunin1, Jeroen Raes, J Kirk Harris
1Microbial Ecology Program, DOE Joint Genome Institute, Walnut Creek, CA, USA.
Abstract:
To investigate the extent of genetic stratification in structured microbial communities, we compared the metagenomes of 10 successive layers of a phylogenetically complex hypersaline mat from Guerrero Negro, Mexico. We found pronounced millimeter-scale genetic gradients that were consistent with the physicochemical profile of the mat. Despite these gradients, all layers displayed near-identical and acid-shifted isoelectric point profiles due to a molecular convergence of amino-acid usage, indicating that hypersalinity enforces an overriding selective pressure on the mat community.
Related Concept Videos
Microbial Mats
Deep Sea Microbial Ecology
Marine Microbial Ecology
Microenvironments
Modern Molecular Taxonomy
Evolution of Microbial Genome

