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Related Experiment Video

Updated: Jul 4, 2026

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

Synaptic vesicle protein 2 binds adenine nucleotides.

Jia Yao¹, Sandra M Bajjalieh

¹Department of Pharmacology, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA.

The Journal of Biological Chemistry

|June 6, 2008

Summary

Synaptic vesicle protein 2 (SV2) binds adenine nucleotides, suggesting a link between cellular energy and regulated secretion. This finding reveals SV2

Area of Science:

Neuroscience
Molecular Biology
Biochemistry

Background:

Synaptic vesicle protein 2 (SV2) is crucial for calcium-regulated secretion of hormones and neurotransmitters.
SV2 isoforms (SV2A, SV2B) are essential for maintaining the readily releasable pool of synaptic vesicles, indicating a role in vesicle priming.

Purpose of the Study:

To investigate the potential ATP-binding properties of SV2, given the presence of putative ATP-binding sites.
To explore the interaction of SV2A and SV2B with nucleotides and identify potential binding sites.

Main Methods:

Utilized a photoaffinity reagent (8-azido-ATP[gamma] biotin) to examine nucleotide binding to purified, recombinant SV2A and SV2B.
Tested binding in the presence and absence of various nucleotides to determine affinity and specificity.

More Related Videos

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

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An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

Published on: June 26, 2018

Related Experiment Videos

Last Updated: Jul 4, 2026

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

Published on: June 26, 2018

Main Results:

SV2A and SV2B were confirmed to bind nucleotides, with a preference for adenine-containing nucleotides.
Two nucleotide-binding sites were identified within the cytoplasmic domains of SV2A, preceding transmembrane domains 1 and 7.

Conclusions:

SV2-mediated vesicle priming may be regulated by adenine nucleotides.
This nucleotide interaction provides a potential mechanism linking cellular energy status to regulated secretion processes.