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Updated: Jul 4, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Improved high-throughput ultrafiltration process enables cRNA purification for gene expression profiling.

Mark D Morris¹, Michael R Meyer, Gary Painchaud

¹Rosetta Inpharmatics LLC, Seattle, WA 98109, USA. mark_morris@merck.com

Assay and Drug Development Technologies

|June 6, 2008

Summary

This summary is machine-generated.

This study presents an improved ultrafiltration method for purifying cRNA, enhancing sensitivity for sensitive RNA applications. The novel protocol avoids chaotropic salts, offering a valuable alternative for nucleic acid purification.

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High-throughput Purification of Affinity-tagged Recombinant Proteins

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Published on: August 26, 2012

Area of Science:

Molecular Biology
Biotechnology

Background:

Ultrafiltration is a common technique for nucleic acid purification.
Existing methods for cRNA purification have limitations in sensitivity.

Purpose of the Study:

To develop a novel ultrafiltration process for purifying cRNA products.
To enhance the sensitivity and signal-to-noise ratio for downstream applications.

Main Methods:

Modification of the ultrafiltration process.
Inclusion of proteinase K preincubation.
Incorporation of a phosphate buffer wash.

Main Results:

The modified ultrafiltration process significantly increased sensitivity and signal-to-noise ratio.
The new protocol eliminates the need for chaotropic salts.
Demonstrated good performance for sensitive RNA applications.

Conclusions:

The developed ultrafiltration technique provides a sensitive and effective method for cRNA purification.
This protocol offers an alternative to silica-based methods, avoiding chaotropic salts.
The technique may be applicable to a wider range of nucleic acid purification needs.