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Updated: Jul 1, 2026
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
Published on: February 27, 2015
High-throughput proteomics of breast carcinoma cells: a focus on FTICR-MS
Arzu Umar1, Malgorzata Jaremko, Peter C Burgers
1University Medical Center Rotterdam, Josephine Nefkens Institute, Laboratory of Genomics & Proteomics in Breast Cancer, Department of Medical Oncology, Dr. Molewaterplein 50, Be 430c, PO Box 2040, 3000 CA, Rotterdam, The Netherlands. a.umar@erasmusmc.nl
Abstract:
Discovery of better biomarkers for diagnosis, prognosis and therapy-response prediction is the most critical task of a scientific quest aimed at developing novel, tailormade therapies for patients with cancer. Consequently, a proteome-wide analysis, in addition to genomic studies, is an absolute requirement for a complete functional understanding of tumor biology. Ultra-sensitive, high-performance Fourier-transform ion-cyclotron resonance (FTICR) mass spectrometry (MS) currently holds an important role in fulfilling the demands of biomarker discovery. In this review, we describe the applicability of FTICR-MS for breast cancer proteomics, particularly for the analysis of complex protein mixtures obtained from a limited number of cells typically available from clinical specimens.
