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Updated: Jul 4, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Lab-on-a-chip technologies for proteomic analysis from isolated cells.

H Sedgwick¹, F Caron, P B Monaghan

¹Department of Electronics and Electrical Engineering, University of Glasgow, Glasgow G12 8QQ, UK. hs@elec.gla.ac.uk

Journal of the Royal Society, Interface

|June 7, 2008

Summary

This summary is machine-generated.

This study presents a novel lab-on-a-chip device for single-cell analysis. The microfluidic system successfully isolates, electroporates, and lyses individual cells for protein release studies.

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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Published on: October 15, 2013

A Method of Targeted Cell Isolation via Glass Surface Functionalization

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

Area of Science:

Biomedical Engineering
Cell Biology
Microfluidics

Background:

Lab-on-a-chip (LOC) systems enable precise manipulation of small sample volumes.
Single-cell analysis is crucial for understanding cellular heterogeneity and disease mechanisms.

Purpose of the Study:

To develop an integrated microfluidic device for single-cell isolation, electroporation, and lysis.
To demonstrate the capability of the LOC system for analyzing protein release from single cells.

Main Methods:

A microfluidic device with saw-tooth microelectrodes was designed and fabricated.
Dielectrophoresis was used to trap A431 human epithelial carcinoma cells.
On-chip electroporation was performed using the integrated electrodes to induce cell lysis.
Confocal fluorescence microscopy monitored protein release post-lysis.

Main Results:

Successful isolation and trapping of single A431 cells were achieved using dielectrophoresis.
Integrated on-chip electroporation effectively induced cell lysis.
Confocal microscopy visualized and confirmed protein release from lysed single cells.

Conclusions:

The developed lab-on-a-chip device provides an integrated platform for single-cell manipulation and analysis.
This technology facilitates efficient cell lysis and subsequent molecular analysis, such as protein release.
The system holds potential for advancing high-throughput single-cell studies in various biological and medical applications.