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Updated: Jul 4, 2026

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
Published on: October 15, 2014
[Blood culture analysis in children with central venous catheter]
Anna Delińska-Galińska1, Elzbieta Arłukowicz, Katarzyna Plata-Nazar
1Klinika Pediatrii, Gastroenterologii i Onkologii Dzieciecej Akademii Medycznej w Gdańsku. agal@amg.gda.pl
Insights
Central venous catheters in children significantly increase the risk of bloodstream infections, with Staphylococcus epidermidis being the most common pathogen. Multi-resistant strains were prevalent, especially in intensive care units.
Area of Science:
- Infectious Diseases
- Pediatrics
- Microbiology
Context:
- Central venous catheters (CVCs) are crucial in pediatric care but pose infection risks.
- This study investigated bloodstream infections in hospitalized children with CVCs.
Purpose:
- To determine the incidence and types of bacteria in blood cultures from children with CVCs.
- To compare infection rates between children with and without CVCs.
Summary:
- Positive blood cultures were more frequent in children with CVCs (57.5%) versus those without (7.4%).
- Staphylococcus epidermidis was the most common bacterium identified.
- Multi-resistant strains, particularly methicillin-resistant coagulase-negative staphylococci, were frequently isolated, especially from intensive care unit patients.
Impact:
- Highlights the significant risk of CVC-associated bloodstream infections in pediatric patients.
- Underscores the need for vigilant infection control measures in pediatric intensive care settings.
- Informs clinical practice regarding antibiotic stewardship and catheter management in children.
Abstract:
We analyzed 99 blood cultures taken from 28 children with central venous catheter. Children were hospitalized in pediatric, pediatric surgery and pediatric intensive care department. All samples were collected from peripherial vein. Positive blood cultures were obtained more frequently from children with central venous catheter than from children without central venous catheter (57.5% vs. 7.4%). Staphylococcus epidermidis was the most frequently obtained bacteria. The other bacterial species were obtained less frequently. The highest percentage of multi resistant straines was isolated from blood samples collected from intensive care department patients. In each departments in which coagulase-negative Staphylococci were isolated, metycillin-resistant straines dominated.
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