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Updated: Jul 4, 2026

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
Published on: August 25, 2022
Reconstitution of bacteriorhodopsin into cyclic lipid vesicles
Motonari Shibakami1, Hisayoshi Tsuihiji, Shin Miyoshi
1Institute of Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566, Japan. moto.shibakami@aist.go.jp
Abstract:
Bacteriorhodopsin (bR), a membrane protein that can generate a light-driven proton pump, was successfully reconstituted into vesicles composed of an artificial cyclic lipid that mimics archaeal membrane lipids. Unlike reconstituted bR in 1,2-dimyristoyl-sn-glycero-3-phosphocholine vesicles, the net topology and structure of bR molecules in cyclic lipid vesicles are identical to those in the native purple membrane of Halobacterium salinarum.

