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Updated: Feb 5, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Published on: July 17, 2021
A bicistronic baculovirus vector for transient and stable protein expression in mammalian cells
Andreas Lackner1, Kathrin Genta, Herwig Koppensteiner
1Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, A-1090 Vienna, Austria.
Abstract:
Baculoviruses are widely used for protein production in insect cells, and their potential for gene transfer to mammalian cells is increasingly being recognized. Here we describe a baculovirus vector with a bicistronic mammalian expression cassette and demonstrate its suitability for efficient transient and stable protein expression in human glioblastoma cells. Bicistronic baculovirus vectors are safe, cost efficient, and easy to produce; thus, they represent an excellent gene transfer system for mammalian cells.
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