Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 4, 2026

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
06:19

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

High throughput pH optimization of protein crystallization.

Ran Meged1, Orly Dym, Joel L Sussman

  • 1Israel Structural Proteomics Center (ISPC), Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA Mechanical Strain Steers Transcription Factor Recognition.

Research square·2026
Same author

Systematic DNA nicking reveals the structural logic of protein recognition.

bioRxiv : the preprint server for biology·2025
Same author

Complete computational design of high-efficiency Kemp elimination enzymes.

Nature·2025
Same author

A cryo-electron microscopy structure of yeast Pex5 in complex with a cargo uncovers a novel binding interface.

Journal of cell science·2025
Same author

Cathepsin X is a conserved cell death protein involved in algal response to environmental stress.

Current biology : CB·2025
Same author

CARD domains mediate anti-phage defence in bacterial gasdermin systems.

Nature·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Automated systems can now optimize crystallization conditions for protein structure determination using x-ray crystallography. This protocol enables efficient pH and ionic strength grid construction for improved crystal growth.

Area of Science:

  • Structural biology
  • Biophysics
  • X-ray crystallography

Background:

  • High-throughput structural proteomics commonly employs the sitting-drop method for macromolecular crystallization.
  • Existing robotic systems primarily support initial screening, not condition optimization.

Purpose of the Study:

  • To describe a protocol for automating the optimization of crystallization conditions.
  • To enable efficient construction of pH optimization grids for crystal screening.

Main Methods:

  • Utilizes automated systems for dispensing crystallization conditions.
  • Implements a protocol for constructing pH optimization grids with varying ionic strengths.
  • Employs fixed buffer sets for systematic condition variation.

More Related Videos

High-Throughput Protein Crystallization via Microdialysis
06:18

High-Throughput Protein Crystallization via Microdialysis

Published on: March 3, 2023

Optimization of Crystal Growth for Neutron Macromolecular Crystallography
12:29

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Published on: March 13, 2021

Related Experiment Videos

Last Updated: Jul 4, 2026

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
06:19

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

High-Throughput Protein Crystallization via Microdialysis
06:18

High-Throughput Protein Crystallization via Microdialysis

Published on: March 3, 2023

Optimization of Crystal Growth for Neutron Macromolecular Crystallography
12:29

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Published on: March 13, 2021

Main Results:

  • Demonstrates a protocol for automated optimization of sitting-drop crystallization.
  • Facilitates the creation of pH and ionic strength grids directly in crystallization plates.
  • Enhances the efficiency of identifying optimal crystallization conditions.

Conclusions:

  • Automated systems can be effectively used for crystallization condition optimization.
  • The described protocol streamlines the process of structural proteomics.
  • Improved crystal quality and structure determination are anticipated.