A CK19(CreERT) knockin mouse line allows for conditional DNA recombination in epithelial cells in multiple endodermal

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Anna L Means1, Yanwen Xu, Aizhen Zhao

  • 1Program in Developmental Biology and the Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Genesis (New York, N.Y. : 2000)
|June 11, 2008
PubMed

Insights

Researchers developed a new K19(CreERT) mouse model for studying gene function and cell lineage. This tamoxifen-inducible system allows for precise genetic manipulation in multiple organs, aiding research in development and regeneration.

Area of Science:

  • Developmental Biology
  • Genetics
  • Regenerative Medicine

Background:

  • Cre/LoxP recombination is crucial for gene function and cell lineage studies.
  • Existing models may lack specificity or broad applicability across multiple organ systems.

Purpose of the Study:

  • To derive and characterize a novel K19(CreERT) mouse line for inducible genetic manipulation.
  • To enable precise cell lineage tracing and gene function analysis in epithelial tissues.

Main Methods:

  • Knock-in of tamoxifen-activable CreERT into the endogenous cytokeratin 19 locus.
  • Administration of tamoxifen to induce DNA recombination.
  • Analysis of Cre reporter expression in various epithelial tissues.

Main Results:

  • The K19(CreERT) mouse line exhibits minimal leaky Cre activity without tamoxifen.
  • Tamoxifen administration induces dose-dependent recombination in stomach, intestine, pancreas, and liver epithelial cells.
  • Cre activity is successfully induced in long-term epithelial stem/progenitor cells.

Conclusions:

  • The K19(CreERT) mouse line is a valuable tool for genetic manipulation and cell lineage tracing.
  • This model facilitates studies of tissue homeostasis and regeneration across multiple organs.
  • The system allows for targeted gene activity modulation in epithelial stem/progenitor populations.

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