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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Dynamic Single-Binding Event Profiling With on-Chip Microlenses for Wash-Free Digital Biosensing.

Tingting Zhan^1,2, Lianyu Lu¹, Guoqiang Gu¹

¹Research Center For Biosensing and Intelligence, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|June 28, 2026

Summary

This summary is machine-generated.

This study introduces a microlens-assisted biosensor for rapid, sensitive single-molecule detection. The platform enables wash-free, kinetic monitoring of biomarkers like cardiac troponin I for point-of-care diagnostics.

Keywords:

digital immunoassay microlens chip plasmonic nanoparticle single‐binding event

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Published on: September 23, 2013

Area of Science:

Biomedical Engineering
Nanotechnology
Analytical Chemistry

Background:

Conventional assays struggle with long incubation times and nonspecific binding.
Kinetic assays offer real-time monitoring but require complex optics, limiting point-of-care use.
There is a need for portable, user-friendly biosensors with high sensitivity and specificity.

Purpose of the Study:

To develop a microlens-assisted platform for kinetic single-molecule detection using conventional microscopy.
To enable wash-free digital biosensing with high sensitivity and specificity.
To demonstrate the platform's utility for point-of-care diagnostics.

Main Methods:

Utilized an on-chip microlens array to amplify scattering from gold nanoparticle labels.
Employed conventional bright-field microscopy for real-time tracking of single-binding events.
Analyzed dynamic fingerprints of binding events to differentiate specific from nonspecific interactions.

Main Results:

Achieved kinetic monitoring with low-magnification optics and a compact optical configuration.
Demonstrated wash-free digital biosensing with high sensitivity and specificity.
Detected cardiac troponin I at an ultralow limit of 0.051 pg mL^-1 in undiluted serum samples.

Conclusions:

The microlens-assisted platform provides a portable and user-friendly solution for kinetic biosensing.
This technology enables laboratory-grade diagnostics at the point of need, improving acute myocardial infarction detection.
The approach overcomes limitations of conventional assays and complex kinetic setups, paving the way for advanced point-of-care devices.